Universitat Internacional de Catalunya

Tissue Engineering Lab

Tissue Engineering Lab
6
13568
3
Second semester
OB
ADVANCED TRAINING
MEDICINE III
Main language of instruction: English

Other languages of instruction: Catalan, Spanish

Teaching staff

Introduction

The combination of biological materials (biomolecules and cells) and biomaterials (synthetic or natural) in the field of bioengineering has proved to be an effective tool for the regeneration and / or functional repair of tissues. Therefore, the design and manufacture of biomaterials and their characterization, both physical and biological, are very important when obtaining certain properties necessary for their application in order to favor these regenerative processes and therefore restore the functionality of the tissue.

Pre-course requirements

It is recommended that the student has successfully completed the course of Tissue Engineering (3rd year, 1st semester).

Objectives

• To know cell culture techniques

• To manufacture 3D scaffolding based on different materials

• To characterize (physically-chemically and biologically) the properties of these scaffolds

• To graph and analyze the results

• To perform laboratory reports

• To  explain and discuss the differnt topics and results

Competencies

  • CB1 - Students must demonstrate that they have and understand knowledge in an area of study based on general secondary education. This knowledge should be of a level that, although based on advanced textbooks, also includes some of the cutting-edge elements from their field of study.
  • CB3 - Students must have the ability to bring together and interpret significant data (normally within their area of study) and to issue judgements that include a reflection on important issues that are social, scientific or ethical in nature.
  • CB5 - Students have developed the necessary learning skills to undertake subsequent studies with a high degree of autonomy.
  • CE6 - To incorporate the foundations of science and materials technology, while taking into account the relationship between microstructure, synthesis or process and the properties of materials.
  • CG10 - To know how to work in a multilingual and multidisciplinary environment.
  • CG3 - To be able to learn new methods and theories and be versatile so as to adapt to new situations.
  • CT5 - To use information sources in a reliable manner. To manage the acquisition, structuring, analysis and visualisation of data and information in your specialist area and critically evaluate the results of this management.

Learning outcomes

• To know and implement the different techniques for the production of biomaterials

• To know and put into practice the basic methods of cell culture, both in 2D and 3D

• To know basic techniques for the characterization of andiamos 3D and analyze its properties

• To be able to graphically and interpret the results using software programs, as well as to make scientific reports

• To be able to interpret and reason the results, present and defend their arguments through group discussion

• To know and implement the mode and dynamics of teamwork

• To be able to function in a scientific laboratory and prepare scientific reports on the topics to be developed

Syllabus

1. Basic cell culture techniques

2. Manufacture of 3D scaffolding using conventional techniques and 3D printing

3. Manufacture of cellular microcarriers

4. Physical-chemical characterization of 3D scaffolding

5. Biological characterization of 3D scaffolding

6. Study of cell-material interactions

Teaching and learning activities

In person



The subject will be divided by topics that could include one or several sessions in relation to the different contents. Each session will consist of a previous theoretical explanation, an experimental part, analysis of the results and a group discussion.

 

During the sessions, students will work on a laboratory notebook where they will write down calculations, data and notes needed during the laboratory session. At the end of each session, the notebook will be supervised and stamped by the teacher.

 

Students will also have to present their results and conclusions through slide presentations for an open group discussion.

 

In addition to the teamwork that will be carried out in each session, students will have to write a laboratory report and hand it to the teacher for the motivation of their autonomous learning. Eventually, the teacher could use the Moodle platform that could include various resources, such as forms, exercises, multimedia material ... that the student must perform to complete the subject.

 

Classes will be taught in English, although students' questions will be answered in the language of their choice (Spanish, Catalan or English). In addition, the student can choose to perform the exercises, assignments and exams in any of these languages. The teaching material will be presented mainly in English, with some exceptions (graphs, tables ...)

 

Students may use calculator and formulary file during exams. The formulary file may only contain formulas, not explanations.

 

The list of ECTS credits and the workload in learning hours depending on the different methodologies that will be used. Each ECTS theoretical credit has 10 hours in which the teacher has a presence in the classroom. The rest of the hours up to 25 correspond to the load of directed and autonomous learning of the student. This last teaching load can be done through autonomous activities, group work that will be presented and defended in class or individual study necessary to achieve the learning objectives of the different subjects.

Evaluation systems and criteria

In person



First call exam:

  1. Laboratory report (35%)
  2. Laboratory notebook (30%)
  3. Final exam (35%)

 

 

Without submitting the practice reports, the subject cannot be passed.

 

Second call exam: Only the note of said exam will be taken into account. In addition, there will be no option for honors registration.

 

Important considerations

  1. Plagiarism, copying or any other action that may be considered cheating will be zero in that evaluation section. Besides, in exams it will mean the immediate failing of the whole subject.
  2. Changes of the calendar, exam dates or the evaluation system will not be accepted. Exchange students (Erasmus and others) or repeaters will be subjected to the same conditions as the rest of the students.
  3. Attendance must be higher than 80% to pass the subject.
 

The student will have to bring a laboratory notebook, lab coat and safety glasses by laboratory.

Bibliography and resources

Johnna S. Temenoff & Antonios G. Mikos. Biomaterials: The intersection of Biology and Materials Science. Pearson, 2009