Universitat Internacional de Catalunya
Biotechnology
Other languages of instruction: Catalan, English
Teaching staff
Questions will be resolved in person or by videoconference with the corresponding tutor.
If necessary, make an appointment with the course coordinator: Dr Naroa Uria.
Introduction
Biotechnology is defined by the use of biological organisms to obtain products or processes with particular purposes, as well as the development of biology-based technology such as the recent CRISPR-Cas9.
This subject will cover both the medical aspects of biotechnology, in terms of gene therapy and the development of pharmaceutical and health products (vaccines, antibiotics), as well as other fields in biotechnology, such as the important role it plays in the food and environmental sectors. It will delve into the millions of applications and tools that biotechnology provides to the medical field and to society in general.
Pre-course requirements
To be able to enrol on this course, it is recommended to have passed:
• Cell biology I
• Cell biology II
• Molecular biology
• Genetics
• Genetic engineering
• Microbiology
Objectives
1. To connect the origins of biotechnology to the development of gene therapies.
2. To connect biotechnology to different industries for the benefit of humans.
3. To understand the contributions of biotechnology to different sectors such as production processes (proteins, fermentation products, etc.), diagnostic systems and to detect or check for diseases.
4. To understand the development of vaccines, antibiotics and peptides.
5. To see biotechnology as a source of energy.
Competences/Learning outcomes of the degree programme
Basics and generals:
• CB3: That the students have the capacity to gather and interpret relevant matters (normally dins of the seva area of study) per a emetre judicis that includes a reflection on relevant issues of a social, scientific or ethical nature.
• CG3: Use and critically assess biomedical techniques.
• CG7: Integrate the basic concepts related to the field of biomedicine both at the theoretical and experimental level.
Specific:
• CE16: Identify and know how to apply the instrumental and experimental techniques of the disciplines of the Biomedical Sciences area, així with the technology related to the biomedical, health and industrial area.
Cross-cutting competencies:
• CT3: Develop the capacity of analysis and synthesis.
• CT4: Interpret experimental results and identify elements that are consistent and inconsistent.
• CT7: Be able to work in teams.
• CT8: Raise and assess the situations and results from a critical and constructive point of view.
• CT11: Apply theoretical coneixements to practice.
• CT12: Apply the scientific method.
Learning outcomes of the subject
The student:
• Will be able to describe the bases of the design and operation of biotechnological processes and identify different processes and products.
Syllabus
Unit 1. Introduction to biotechnology. Defining the objective of biotechnology. Origin of biotechnology and bioprocesses. Recombinant DNA technology and gene therapy. Applications. (1)
Unit 2. Microbial biotechnology. Microorganisms of industrial interest: diversity, isolation, selection and maintenance. Improving and developing of strains in industrial microbiology. Bioprocesses. Microbial products of industrial interest. (2)
Unit 3. Animal biotechnology. Reproductive biotechnology and animal production, Types of transgenic animals: how they are developed and their applications, Animals as bioreactors. (1)
Unit 4. Medical biotechnology. Tissue engineering: application in the medical industry of the cultivation and use of artificial tissues. Lignans and coumarins: pharmaceutical applications to combat cancer and antithrombotic agents. (1)
Unit 5. Plant biotechnology. Obtaining transgenic plants and the current situation of transgenic plants. Plants as biofactories. Applications of plant genetic engineering to improve the quality of plant products. Transgenesis for resistance to biotic and abiotic factors. Acetate, fatty acid and prostaglandins production. Terpenoids and steroids of industrial interest. (2)
Unit 6. Development and formulation of pharmaceutical products. Concept and general characteristics, Evolution and current trends, Development, production and market costs. (1)
Unit 7. Vaccines. Concept and origin of vaccines, Types of vaccines and mode of action, Inactive, attenuated, native subunits, recombinants, nucleic acids, toxoids..., Composition of vaccines, adjuvants, stabilizers and preservatives.(1)
Topic 9. Environmental biotechnology. Biofuels, Bioelectrogenesis, Production of biopolymers, treatment of liquid and solid waste. (1)
Topic 10. Bioremediation. Synthesis of herbicides and insecticides. Microbial fertilisers. Biological control. (1)
Unit 11. Nanotechnology. Systems with extended biological life. Systems that facilitate ligant-receptor interactions. Systems with the ability to respond to stimuli. Controlled release. (1)
Unit 12. Biosensors and Biochips. Classification. Biomarkers. Applications. Rapid diagnosis systems. DNA chips.
Unit 12. Biosensors and Biochips. Classification. Biomarkers. Applications. Rapid diagnostic systems. DNA chips. Protein microarrays. (1)
Topic 13. Legal and social aspects of biotechnology. Use of transgenic plants to improve food production. In vitro meat. Genes on demand. (1)
Case methods:
MC1: Microbial Biotechnology: Industrial Enzymes MC2: Plant Biotechnology: biofortified crops MC3: Animal Biotechnology: Animal Reproductive Biotechnology MC4: Bioterrorism MC5: Forensic biotechnology. genetic footprint MC6: Environmental Biotechnology: Microbial Fuel Cells MC7: Patents in Biotechnology
Teaching and learning activities
In person
Lectures: 50-minute presentation of a theoretical topic by the lecturer. Visual support in powerpoint format used to accompany the explanations.
Case studies or case methods (CM): Approaches to a real or imaginary situation. Students work on questions in small groups or in active interaction with the lecturer and the answers are discussed. The lecturer intervenes actively and, if necessary, contributes new knowledge. These classes may cover content that goes into further detail on the topics covered in masterclasses or may look at new topics. The case methods as just as important and have the same weight as the questions in the final exam.
Online Education (OE): Online material located on the Moodle platform that the student can consult from any computer, at any time and that will contribute to the self-learning of concepts related to the subject. The online material is part of the content for the course and will be evaluated in the final exam.
Laboratory Practice (LP): Practical sessions that work on the experimental techniques covered in the syllabus in the theoretical classes. Familiarisation with industrial fermentation to manufacture products for human consumption. Attendance is compulsory and the content of the practical sessions will be evaluated at the end of the practical sessions.
Evaluation systems and criteria
In person
1) Assessment:
• Mid-term exam: 20%
• Resolution of case methods: 15%
• Laboratory practical exam: 10%
• Final exam: 55%
Teaching staff reserve up to 10% of the final mark to be awarded for subjective matters such as: involvement, participation, respect for basic rules, etc.
2) Retakes: same criteria.
3) Repeating students: the grades awarded during continuous assessment (participation, case methods, practices) will be maintained, although whenever they wish, students will be able to repeat the course and obtain a new grade. Mid-term grades will not be maintained.
General points to keep in mind about the evaluation system:
1) In the final exam, a minimum grade of 5 must be obtained to calculate the final grade including the continuous assessment grades (case methods and mid-term exams). The exams will be multiple choice with 4 options, with +1 for correct answers and -0.25 for errors.
2) Both class participation and laboratory practice will be evaluated through short Multiple choice exams.
3) 10% of the questions in the exams could be about concepts that have not been explained directly in the classroom, but that are covered in the recommended bibliography as well as in discussed articles, press or recommended online material.
4) Class attendance:
• Regular attendance at theoretical sessions and case methods classes is recommended.
• Attendance at masterclasses is not compulsory, but attendees will be governed by the rules indicated by the lecturers. The expulsion of a student from a masterclass or case method will have a negative impact on continuous assessments.
• Attendance to case methods is optional. The cases evaluated will be averaged proportionally.
• Attendance at practical sessions is compulsory and students must attend in the assigned groups. The expulsion of a student from the laboratory will mean they automatically fail the course.
5) When granting honours, the candidates will have special consideration for their participation and involvement in the different methodologies in the course, as well as their respect for basic rules.
6) The improper use of electronic devices, such as mobile phones, tablets or laptops, may lead to expulsion from class. Misuse is understood to be recording and sharing both the students or lecturers during classes, as well as the use of these devices for recreational and non-educational purposes.
Bibliography and resources
Katoh S. y Yoshida F. (2009) Biochemical engineering: a textbook for engineers, chemists and biologists. Editorial Wiley-VCH
Illanes, A. (2008) Enzyme biocatalysis. Principles and applications. Springer.
Liu S. (2017) Bioprocess engineering: Kinetics, Sustainability, and Reactor Design. Editorial Elsevier.
Mutto A., Mucci N., Kaiser G. (2008) Biotecnología aplicada a la reproducción y mejoramiento animal. Editorial Académica Española.
Conn P.M. (2013) Animal Models for the Study of Human Disease. Academic Press.
Kayser O., Warzecha H. (2012) Pharmaceutical Biotechnology: Drug Discovery and Clinical Applications (2nd Edition). Wiley-Blackwell.
Vallero D. (2015) Environmental Biotechnology: A Biosystem Approach. Academic Press.
Yang S-T. (2007) Bioprocessing for value-added products from renewable resources. Ed. Elsevier.
Thieman W., Palladino M. (2012) Introduction to Biotechnology. Pearson Education Limited.
Evaluation period
- E1 10/01/2023 I3 14:00h